Comprehensive analysis of phenolic composition and antioxidant  mechanisms in Gymnema sylvestre extracts using LC-MS and column chromatography

Asian Journal of Traditional Medicines ›› 2024, Vol. 19 ›› Issue (4): 211-222.

**Comprehensive analysis of phenolic composition and antioxidant mechanisms in Gymnema sylvestre extracts using LC-MS and column chromatography**

Online:2024-09-19 Published:2024-09-20

Abstract

Abstract:

Recent studies have highlighted the potential of plant extracts as therapeutic agents for managing oxidative stress and related disorders. This study aims to elucidate the phenolic composition and antioxidant properties of Gymnema sylvestre extracts. Ethanolic reflux extraction followed by column chromatography was employed to isolate phenolic compounds. The total phenolic and flavonoid contents were quantified using the Folin–Ciocalteu and aluminum chloride colorimetric methods, respectively. Antioxidant activities were assessed by DPPH, ABTS scavenging assays and the ferric reducing antioxidant power (FRAP) assay. High-Performance Liquid Chromatography (HPLC) with a C18 column and Thermo TSQ Quantum Access Max (LC-MS) were used to determine the levels of gymnemic acid and identify other potential phenolic compounds. The analysis revealed significant antioxidant activities in the fractions. Fraction A showed the highest DPPH and ABTS scavenging activities, and Fraction C demonstrated the highest ferric reducing power. LC-MS analysis identified several phenolic compounds, indicating that these are major contributors to the antioxidant efficacy of the extract. This study provides a detailed phenolic profile and confirms the strong antioxidant potential of Gymnema sylvestre leaf extract, supporting its therapeutic use and further investigation.

Key words: Gymnema sylvestre; antioxidants, column chromatography, phenolic compounds, LC-MS

Shengjie Hao, Yuxin Du, Jianglong He, Qian Li, Guilin Chen. Comprehensive analysis of phenolic composition and antioxidant mechanisms in Gymnema sylvestre extracts using LC-MS and column chromatography [J]. Asian Journal of Traditional Medicines, 2024, 19(4): 211-222.

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Asian Journal
Of Traditional Medicines