Asian Journal
Of Traditional Medicines

Abstract: Aiming to ensure the consistency of quality control of Traditional Chinese Medicines (TCMs), a combination method of high-erformance liquid chromatography (HPLC), ultraviolet (UV), electrochemical (EC) was developed in this study to comprehensively evaluate the quality of Antiviral Mixture (AM), and Comprehensive Linear Quantification Fingerprint Method (CLQFM) was used to process the data. Quantitative analysis of three active substances in TCM was conducted. A five-wavelength fusion fingerprint (FWFF) was developed, using second-order derivatives of UV spectral data to differentiate sample  levels effectively. The combination of HPLC and UV spectrophotometry, along with electrochemical fingerprinting (ECFP),  successfully evaluated total active substances. Ultimately, a multidimensional profiling analytical system for TCM was developed. 

Key words: TCM, antiviral mixture, five-wavelength fusion fingerprint (FWFF), Comprehensive Linear Quantification Fingerprint Method (CLQFM), quantization fingerprint, antioxidant activity profilling