Identification and quantitative estimation of Emodin in Rhei Emodi Wall. Rhizome and the relevant polyherbal ayurvedic formulation

Abstract

Abstract: The present study explored quality control of plant drug and polyherbal formulation using HPTLC and HPLC fingerprinting. The study combined qualitative analysis of Emodin and Rhein biomarker compound in Rhei Emodi Wall. rhizome extract and polyherbal formulation extract by HPTLC method with quantitative estimation of Emodin by HPLC method, aiming to help in accurate identification of Emodin and Rhein in plant materials, polyherbal formulations. This study also checked adulteration, with the purpose of improving bio-efficacy of ayurvedic formulation. All the samples showed characteristic peaks of Emodin at same retention time as that of standard Emodin. According to the HPTLC fingerprinting results, the Rf values of Rhein reference standard at 0.42 and Emodin at 0.62 were observed in test solution of Rhei emodi Wall. rhizome extract and polyherbal
formulation extract and both were found comparable. The quantitative evaluation of Emodin present in the polyherbal formulation and Rhei emodi Wall. rhizome extracts were 0.0177% and 0.3106% respectively.

Key words: Rhei emodi, Emodin, Rhein, Standardization, HPTLC, HPLC and quantification

Ajay Kumar Meena, R. Ilavarasan, Arjun Singh, Lavkush Sharma, Amit Dixit, N. Srikanth. Identification and quantitative estimation of Emodin in Rhei Emodi Wall. Rhizome and the relevant polyherbal ayurvedic formulation[J]. Asian Journal of Traditional Medicines, 2021, 16(6): 351-366.

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