Asian Journal
Of Traditional Medicines

Abstract: Semen sojae praeparatum is a kind of plant resource used both as medicine and food and has been used in preventing oxidative damage and treating some clinical inflammatory symptoms. However, the involved active mechanisms and the pharmacodynamic material basis of this fermented legume are not clear. Therefore, this study attempts to evaluate the chemical composition and provide further insights of the antioxidant activities of isolated compounds. In the meantime, the anti-inflammatory role and potential mechanisms were investigated. The all results revealed that genistein and it derivatives showed significantly high antioxidant activity. In anti-inflammatory assay, Semen sojae praeparatum extract significantly suppressed acute and chronic inflammation. In vitro results also verified that the extract markedly inhibited inflammatory mediator production at 120 mg/mL. These effects are consistent with previous research about anti-inflammatory activity of flavonoids. Our research also discussed the anti-inflammatory activity of detected constituents on the basis of literature data. It could be concluded that genistein might be showed the most powerful anti-inflammatory activity due to the presence of the phenolic hydroxyl at C-5, C-7 and C-4'. These finding highlight the very powerful antioxidant activity and anti-inflammatory activity of genistein and Semen sojae praeparatum extract, thereby considering its availability as a medicinal or an edible treatment against oxidative and inflammatory diseases.

Key words: UHPLC-Q-TOF-MS, phenolic, isoflavone, anti-inflammatory activity, antioxidant activity