Simultaneous quantification of three major sanshools
in analgesic bacteriostatic gel by HPLC

Simultaneous quantification of three major sanshools in analgesic bacteriostatic gel by HPLC

Rong Rong, Meiyan Zhang, Xianying Zhou, Qili Zhang, Zhiguo Yu, Yunli Zhao*

School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China

Received:2015-03-23 Revised:2016-04-08 Online:2016-04-20 Published:2016-04-08
Contact: Yunli Zhao*, School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, Liaoning 110016, China; Tel: +86-24-23986295; E-mail: yunli76@163.com

Abstract

Abstract: A rapid and sensitive high-performance liquid chromatographic (HPLC) method had been developed and validated for the analysis of three main ingredients in analgesic bacteriostatic gel. Hydroxy-α-sanshool (HAS), hydroxyl-β-sanshool (HBS) and hydroxy-γ-sanshool (HRS) were separated on a Thermo Hypersil GOLD C18 column (150 mm × 4.6 mm, 5 μm) with gradient elution system. The mobile phase consisted of acetonitrile-water (v/v) with a flow rate of 1.0 mL/min and UV detection was performed at 270 nm. The method was validated according to the Chinese Pharmacopeia (2015). Linearity (r > 0.9990) was observed over the concentration ranges of 3.24–58.32, 0.656–11.81 and 0.304–5.48 mg/L for HAS, HBS and HRS. The average recovery for HAS, HBS and HRS was 96.9%, 97.2% and 97.9%. The method was found simple and rapid, which can be suitable for the determination of the three main ingredients in analgesic bacteriostatic gel.

Key words: analgesic bacteriostatic gel, sanshools, Method validation, HPLC

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