Asian Journal
Of Traditional Medicines

Abstract: In this paper, a novobiocin super sensitive mutant Mycobacterium phlei S2 and a super resistant mutant M. phlei R2 were first obtained with the UV mutagenesis method. Based on the susceptibility differences among M. phlei 1180, M. phlei S2 and M. phlei R2, an antituberculosis primary screening model was established. Whether the target specificity (DNA gyrase subunit B) screening model was suitable for screening the drugs was investigated using the paper disk method. With the application of the screening model, three traditional medicine extracts were selected having obvious target specificity effect.

Key words: Mycobacterium phlei, DNA gyrase, Screening model, Traditional medicines