Asian Journal
Of Traditional Medicines

Abstract: High-performance liquid chromatographic fingerprint technique was adopted to monitor the quality of traditional Chinese
medicine (TCM) Xue Fu Zhu Yu Pill (XFZYP). The novel 24 levels of systematic quantified fingerprint methods (SQFMs)
were set up, and 230 nm HPLC fingerprints as well as 6 maker components were determined and identified, in combination of
antioxidant activity detected by DPPH. Chromatographic fingerprints resolution information index RI was employed to screen
the optimized condition for sample analysis, and chemometrics was used to extract more information from the data. By 24 levels
SQFMs, the samples show accepted quality of 1~4. With help of multi-criteria decision making ranking methods (MCDM)
PROMETHEE (Preference Ranking Organization Method for Enrichment Evaluation) and GAIA (Geometrical Analysis for
Interactive Aid), the XFZYP samples from two manufactures were efficiently differentiated and classified. Furthermore, the
antioxidant activity results expressed as IC50 were further analyzed by PLSR (Partial Least Squares Regression) to establish
spectrum-efficacy relationship and to predict the IC50. The work indicates that the proposed method is sensitive, efficient and
reliable for fast determining complex chemicals, which is practical and possible for controlling quality of XFZYP.

Key words: Xue Fu Zhu Yu Pill, 24 levels of SQFMs, antioxidant activity, RI, PROMETHEE and GAIA, PLSR