Asian Journal
Of Traditional Medicines

Abstract: Quercetin is a polyphenolic naturally occurring compound potentially having a huge impact in human benefit which includes
wound healing, cardiovascular, carcinoma therapeutic activities as well as have nutraceutical benefits. The objective of study was
to establish a precise, accurate, sensitive and reliable method for estimation of low concentrations of quercetin in biological fluids,
using spectrofluorimetric method. A standard curve of quercetin was prepared in methanol and serum in the concentration
range 1-10 ng/ml. The method was validated in terms of linearity, accuracy and precision. The regression data for quercetin in
methanol and serum observed to have a good linear relationship with R2 > 0.98 over the concentration range of 1-10 ng/ml. This
method gave adequate linearity, precision, accuracy and recovery. It is also concluded that method is less time consumption as
compared with HPLC, which requires lengthier chromatographic separation.

Key words: quercetin, spectrofluorimetric, accuracy, precision