Asian Journal
Of Traditional Medicines

Abstract: Chromatographic normal and reversed fingerprints coupled with Linear System Quantified Fingerprints Method (LSQFM)
were proposed for quality control of traditional Chinese medicine (TCM) by high performance liquid chromatography (HPLC)-
photodiode array detection. The method was performed to evaluate the quality of Yinhuang Tablet (YHT). Statistical analysis of
the obtained data demonstrated that our method has achieved ideal precision and accuracy. Normal and reversed fingerprints
analysis can monitor both the contribution of high content compositions and low or trace constituents, which are rarely
investigated because their contents are low, their preparation and characterization are difficult. Due to the different contribution
of the eight biggest and smallest peaks in the normal and reversed fingerprints, a few batches of samples had the intuitive
contrast of SL, ML, α and quality grade in fingerprint analysis. These results demonstrated that normal and reversed fingerprints
by LSQFM can offer an efficient and reliable approach for quality consistency evaluation of TCM.

Key words: chromatographic normal fingerprint, reversed fingerprint, Linear System Quantified Fingerprints Method, quality control, Yinhuang tablet