Asian Journal
Of Traditional Medicines

Abstract: Two methods based on capillary electrophoresis fingerprints (CEFPs) and infrared spectrum fingerprints (IRFPs) were developed
to quantitatively evaluate the quality of Renshenguipi Wan (RSGPW). The CE conditions, including the CE buffer system
chosen using a triangle optimization method, were optimized using the chromatographic fingerprint resolution index (RF) as the
objective function and according to actual separations achieved. The optimal CE buffer system consisted of 50 mmol/L borax-50
mmol/L disodium hydrogen phosphate (1:1, v/v), with a pH adjusted to 9.5 by phosphoric acid, and the baseline separation was
achieved within 25 min at an applied voltage of 13 kV. A total of 13 common peaks were confirmed to be present in 10 batches of
RSGPWs by choosing ferulic acid as the reference peak and the quality of RSGPW was determined by the system quantitative
fingerprint method (SQFM) on the basis of CEFPs. All samples were extracted with methanol under the same conditions and the
IRFPs of the extracts were recorded. Equal weigh integration of the two methods based on CEFPs and IRFPs was used for the
quality control of RSGPW, which proved that CEFPs combined with IRFPs are better for evaluating the quality of RSGPW.

Key words: Renshenguipi Wan (RSGPW), capillary electrophoresis fingerprints (CEFPs), Infrared spectrum fingerprints (IRFPs), chromatographic fingerprint resolution index (RF), system quantitative fingerprints method (SQFM)