Asian Journal
Of Traditional Medicines

Abstract: The microscopic characters of four species of Folium syringae, Syringa oblata, S. vulgaris, S. diatata and S. chinensis were compared and found the features of the epidermis, mesophyll cells and nerve cells could be used for distinguishing. The HPLC method was applied to determinate the content of dihydroxy benzoic acid of folium syringa, and the linear range of dihydroxy benzoic acid was (0.0315-0.0420) µg, r=0.9992. The recoveries of four species above by this method were 100.29 % (RSD=0.48 %), 101.59 % (RSD=0.71 %), 102.52 % (RSD=3.06 %) and 99.81 % (RSD=2.90 %) respectively. The results demonstrated that the four species of Folium syringae could be discriminated by microscopy, which provide us a simple way to identify crude drug of syringae. Besides, an accurate HPLC method has been established for the determination of dihydroxy benzoic acid, which can be used in the quality control of Folium syringae.

Key words: Folium syringae, microscopic identification, HPLC, dihydroxy benzoic acid