Asian Journal
Of Traditional Medicines

Abstract: A high-performance liquid chromatographic (HPLC) method was developed for the simultaneous determination of four anthraquinones, rhein, emodin, chrysophanol and physcion, in Dangguilonghui Tablets (as traditional Chinese medicine). The optimal conditions involved separation on a Diamonsil-C18 column (200 mm×4.6 mm, 5 μm) with a mobile phase composed of methanol: 0.1 % phosphoric acid (180:20, v/v) and detection at 254 nm. Excellent linearity was obtained over the investigated concentration ranges, with the values of R2 higher than 0.999 for all the analytes. The recoveries, measured at three concentration levels, varied from 99.8 % to 101.2 %. The results indicate that the method is reproducible, precise and can be used for the quality control of Dangguilonghui Tablets

Key words: Dangguilonghui Tablets, rhein, emodin, chrysophanol, physcion, HPLC