Asian Journal
Of Traditional Medicines

Abstract: A simple, sensitive and reproducible high performance liquid chromatography (HPLC) method with ultraviolet (UV) detection has been developed for the determination of terazosin in human plasma. The procedure involved a liquid – liquid extraction of terazosin from human
plasma with ether. Separation was achieved with a reversed-phase C18 column (250 mm × 4.6 mm, 5 μm) employing UV detection at 254
nm. The mobile phase consisted of acetonitrile – tetrahydrofuran - 0.01 mol/l potassium dihydrogenphosphate solution (15:5:80, v/v) at a
flow rate of 1.0 ml/min. The total run time was 8.0 min. The standard curve was linear over a working range of 10 – 400 ng/ml and gave an
average correlation coefficient of 0.9903 during validation. The limit of quantification (LOQ) of this method was 10 ng/ml. The inter-day and
intra-day precisions were between 7.7 % – 2.5 % and 10.3 % – 2.6 %, respectively.The method was found to be precise, accurate and specific
during the study and, hence, it is suitable for use in pharmacokinetic studies of terazosin.

Key words: Terazosin, Human serum, Quantification