亚洲传统医药

Abstract: A simple and rapid gas chromatographic (GC) method with flame ionization detection was developed for the analysis of fatty oil in Periploca sepium. After methyl-esterification, nine components were identified by GC-MS. The derivatization conditions were investigated in order to validate this method. Palmitic acid, oleic acid and linoleic acid were determined simultaneously. The calibration curves were linear in the range of 0.050–0.250 mg·mL-1 for palmitic acid, 0.019–0.190 mg·mL-1 for oleic acid and 0.087–1.044 mg·mL-1 for linoleic acid, respectively. The average recovery of palmitic acid was 99.2 % (RSD = 3.3 %); oleic acid, 99.4 % (RSD = 2.6 %); linoleic acid, 100.2 % (RSD = 2.9 %). The method can be used for the analysis of the fatty acids from Periploca sepium.

Key words: gas chromatography-flame ionization detector, methyl-esterification, fatty acid, Periploca sepium