亚洲传统医药
亚洲传统医药

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›› 2007, Vol. 2 ›› Issue (3): 104-109.

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Constituents from anti-tumor-promoting active part of Dioscorea bulbifera L. in JB6 mouse epidermal cells

  

  • 收稿日期:2007-01-01 修回日期:2007-01-01 出版日期:2007-06-15 发布日期:2007-06-15

Constituents from anti-tumor-promoting active part of Dioscorea bulbifera L. in JB6 mouse epidermal cells

Huiyuan Gao 1; Bailing Hou 1; Masonori Kuroyanagi 2; Lijun Wu 1*
  

  1. 1. School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China 2. School of Bioresources, 560 Nanatsuka, Shobara, Hiroshima Prefectural University, Hiroshima 727-0023, Japan
  • Received:2007-01-01 Revised:2007-01-01 Online:2007-06-15 Published:2007-06-15
  • Contact: Lijun Wu

Abstract: The anti-tumor-promoting effect was found in the 75 % ethanol extracts of the rhizomes of Dioscorea bulbifera L. using the neoplastic transformation assay of mouse epidermal JB6 cell lines. The EtOAc and n-BuOH soluble fractions showed different inhibitory activities against tumor promotion of JB6 (Cl 22 and Cl 41) cells induced by the promoter of 12-O-tetradecanoylphorbol-13-acetate (TPA). The EtOAc and n-BuOH fractions were found to be potent anti-tumor promoters. Twenty-eight compounds were purified in the EtOAc and n-BuOH fractions by chromatography and HPLC and their structures were identified. Among them, compounds 1, 7, 9, 10, 11, 14, 24, 25 and even a mixture of 12, 13 were also examined in the same way on their anti-tumor-promoting activities.

Key words: antitumor promoting activity, JB6 cell, soft agar colony formation method, Dioscorea bulbifera L., chemical constituents

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