亚洲传统医药

本期目录

2021年, 第16卷, 第4期　刊出日期：2021-08-25 上一期    下一期

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Quality assessment of Jinhongtang Granule using UFLC-MS/MS and multivariate statistical analysis Fan Wu, Yu Zhang, Yanling Qiao, Ting Zhao, Baojing Zhang, Bangjiang Fang, Xiaokui Huo, Xiaochi Ma 2021 (4):  191-202.  摘要 ( 498 )   PDF(1138KB) ( 358 )   Jinhongtang is a traditional Chinese medicine formula composed of Rheum palmatum L. stem, Sargentodoxa cuneata stem, and Taraxacum mongolicum and is used for the treatment of sepsis. However, quality assessment method for Jinhongtang is not available. In present study, we developed a UFLC-MS/MS method to determine 16 analytes in 20 batches of home-made and commercial Jinhongtang. Multivariate statistical analysis revealed the significant differences in the quality of home-made and commercial Jinhongtang and the difference in the quality of home-made samples was more significant. The integrated strategy based on UFLC-MS/MS and multivariate statistical analysis provided a new basis for the overall quality assessment of Jinhongtang. 相关文章 | 计量指标

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Microbial transformation for Region-selective Hydroxylation of Z-Butylidenephthalide by Aspergillus niger CGMCC 3.739 Jian Zou, Yongheng Wang, Zhengqun Zhou, Rongrong He, Xinsheng Yao, Hao Gao 2021 (4):  203-209.  摘要 ( 422 )   PDF(2282KB) ( 318 )   Microbial transformation is a useful tool for structure modification of active natural products. Z-Butylidenephthalide is the main active constituent of Angelica sinensis. In the present work, a pair of new oxidized metabolites, namely (–) (11R)-(Z)-11-hydroxybutylidenephthalide (1a) and (+) (11S)-(Z)-11-hydroxy-butylidenephthalide (1b), were obtained from microbial transformation for region-selective 11-hydroxylation of Z-butylidenephthalide by Aspergillus niger CGMCC 3.739. Their structures, including absolute configurations, were elucidated by extensive analysis of HR-ESI-MS, NMR spectra, and the modified Mosher’s method. This is the first study on the microbial transformation of Z-butylidenephthalide, and these findings offer a tool for region-selective 11-hydroxylation of Z-butylidenephthalide. 相关文章 | 计量指标

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Colletodiphenol A, a new phenol derivative isolated from the endophytic fungus Colletotrichum capsici Guiyang Xia, Yanbin Sun, Yuzhuo Wu, Huan Xia, Pengcheng Lin, Sheng Lin 2021 (4):  210-215.  摘要 ( 351 )   PDF(924KB) ( 263 )   A new C6-C2 dimer phenol derivative, colletodiphenol A (1), along with two known C6-C2 phenol derivatives (2,3) were isolated from the endophytic fungus Colletotrichum capsici related to the traditional Chinese medicine Paeonia lactiflora. Their structures were elucidated based on extensive spectroscopic data analysis, including HRESIMS, UV, IR, 1D and 2D NMR. The antioxidant and anti-inflammatory activities of the isolated compounds were evaluated, but none of them displayed significant activity. 相关文章 | 计量指标

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High performance thin layer chromatography as an effective tool for rapid screening of 5α-reductase inhibitors Ruirong Zheng†, Mooseob Kim†, Zhengtao Wang, Li Yang 2021 (4):  216-224.  摘要 ( 357 )   PDF(6290KB) ( 385 )   Benign prostatic hyperplasia (BPH) is a common disease in men, and is known to be related to 5α-reductase, which can affect steroid metabolism. Under the promotion of 5α-reductase, testosterone can be converted into dihydrotestosterone (DHT), and excessive DHT will cause related diseases. Since BPH seriously affects the quality of life of patients, it is essential to discover effective 5α-reductase inhibitors. In this study, the simple analytical method of high performance thin layer chromatography (HPTLC) was used to screen active compounds, and seven compounds with strong activity were screened out. This research will provide a reference for studying the material basis of drugs for the treatment of BPH. 相关文章 | 计量指标

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Isolation and identification of chemical constituents from the vinegar-prepared Corydalis yanhusuo Guiyang Xia, Dongjie Fang, Yuzhuo Wu, Huan Xia, Xinlan Yu, Sheng Lin, Hongcai Shang 2021 (4):  225-233.  摘要 ( 337 )   PDF(886KB) ( 359 )   Phytochemical investigation of the vinegar-prepared Corydalis yanhusuo led to the isolation of one aristolactam derivative, 1,2,8,9-tetramethoxy-5-methyldibenzo[cd,f]indol-4-(5H)-one (1), and seven aporphine alkaloids, including 2,9,10-trimethoxydibenz[de,g]quinolin-7-one (2), 1-hydroxy-2,9,10-trimethoxy-7H-dibenzo(de,g)quinoline-7-one (3), oxoglaucine (4), N-methyloxoglaucine trifluoroacetate trifluoroacetate (5), corunine acetate (6), pontevedrine (7), and oxoglaucidaline trifluoroacetate (8). The structures of the isolated compounds were elucidated by extensive spectroscopic data analysis and comparison with the previous reports. Among them, compounds 1 and 2 were obtained as natural products for the first time, and their NMR data were unambiguously assigned. In addition, compound 1 exhibited moderate cytotoxic activity against HepG2 cells with an IC50 value of 16.0 ± 6.4 μM. 相关文章 | 计量指标

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Network pharmacology-based virtual screening of active alkaloid ingredients from Piper nigrum L. and the potential targets against Parkinson’s disease Ye Chang, Yuxi Wang, Xiaoxiao Huang, Shaojiang Song 2021 (4):  234-239.  摘要 ( 293 )   PDF(2456KB) ( 350 )   Parkinson’s disease (PD) is a common neurodegenerative disease. Despite a great deal of research on this disease, there is still no definitive treatment plan. Literature investigations have shown that Piper nigrum L. has a protective effect on the nerve center. Therefore, network pharmacology was used in this study to investigate the therapeutic potential of P. nigrum for PD. As a result, six active alkaloid ingredients and four potential targets were obtained. This study laid the foundation for finding the lead compounds from P. nigrum to treat PD. 相关文章 | 计量指标

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2-Oxoglutarate-dependent dioxygenases in plants: biosynthesis, mechanism and evolution Lin Ma, Yifeng Zhang, Wei Gao 2021 (4):  240-253.  摘要 ( 371 )   PDF(2270KB) ( 396 )   2-Oxoglutarate (2OG)-dependent dioxygenases (2-ODDs) are omnipresent iron-containing non-heme enzymes that catalyze various oxidation-reduction reactions in plant growth and development, nucleic acid modification and secondary metabolism. We systematically summarized recent research on the oxidative modifications of plant 2-ODDs and related enzymes, their vital importance in the biosynthesis of plant special metabolites, and their catalytic specificity/flexibility, and discussed the potential of 2-ODD as a new approach for the identification of pivotal genes and the elucidation of biosynthetic pathway. 相关文章 | 计量指标