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Main alkaloids of Rhizoma Coptidis improved palmitic acidinduced insulin resistance in HepG2 cells via AMPK and
MAPK signaling pathway
Ming Qu, Ying Wang, Shijie Cao, Yalu Liu, Da Liu, Feng Qiu, Ning Kang
2020 (5):
239-254.
摘要
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Rhizoma Coptidis, a traditional Chinese herbal medicine, has been used for treating diabetes for thousands of years. However,
the molecular basis for this action has not been elucidated. In the present study, the effects of seven main alkaloids of Rhizoma
Coptidis on glycometabolism were investigated and the related molecular mechanism of five active compounds on insulin
resistant (IR) cell model was explored for the first time. Results showed that berberine, palmatine, epiberberine, columbamine
and groenlandicine enhanced glucose consumption in the palmitic acid (PA)-induced IR-HepG2 cells, indicating that these
compounds could improve IR. In addition, we found that among these active alkaloids, berberine, columbamine, epiberberine
and groenlandicine could inhibit the activation of ERK and p38 pathway, while berberine, columbamine, palmatine and
epiberberine could activate AMPK pathway. Moreover, palmatine and columbamine regulated the mRNA expression of GLUT2
to ameliorate IR via activating AMPK and inactivating p38 MAPK signal pathway. To sum up, berberine, columbamine,
palmatine, epiberberine and groenlandicine might be the active reagents, which contribute to the glucose lowering effects of
Rhizoma Coptidis.
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