亚洲传统医药

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2013年, 第8卷, 第6期　刊出日期：2013-12-20 上一期    下一期

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Development of dispersive liquid-liquid microextractionmethod based on solidification of floating organic dropscombined with high performance liquid chromatography for thedetermination of curcumin in rat plasma Linli Wang;Ning Liang;Zhi Sun;Dongjie Liu;Xiaohong Hou* 2013 (6):  147-156.  摘要 ( 523 )   Curcumin is a phenolic compound derived from the rhizomes of Curcuma longa possessing potential anti-inflammatory and anticancer properties. Owing to its poor absorption in blood, sample cleanup and enrichment procedures are required for the analysis of curcumin in plasma. Now, a novel dispersive liquid-liquid microextraction method based on solidification of floating organic drops coupled with high performance liquid chromatography was developed and validated for the pretreatment and determination of curcumin in rat plasma. The important variables such as extraction solvent and volume, dispersive solvent and volume, extraction time, pH, sample volume, and salt concentration, were investigated. Under optimum conditions, 100 μl rat plasma was precipitated with 200 μl methanol then, after centrifuging, 250 μl supernatant was taken and diluted to 3 ml with water and adjusted to a pH of 4. Next, 400 μl disperser solvent and 30 μl extraction solvent were rapidly injected into the sample solution. After vortexing and centrifuging, the sample solution was cooled in an ice bath until the 1-dodecanol was solidified into a small ball. Then, the solidified 1-dodecanol was removed and thawed quickly at room temperature. 100 μl methanol was added before the HPLC analysis. The target analyte was determined over a linear range from 0.01 to 2.0 μg/ml in rat plasma and the limit of detection of the method was 0.003 μg/ml. The enrichment factor and extraction recovery was 20 and 85.6%, respectively. The proposed method was successfully applied to the determination of curcumin in rat plasma. 相关文章 | 计量指标

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A pharmacognostic study of Syringae Cortex Haitao Cheng;Na Han;Zhicheng Zhang;Shouhe Qu;Weining Wang;Jun Yin * 2013 (6):  157-161.  摘要 ( 492 )   It is necessary to study the pharmacognositic characteristics of Syringae Cortex in order to update the content of the Chinese Pharmacopeia. Syringae Cortex was identified by morphological and microscopic identification techniques, and the results obtained are described in detail. This study provides evidence for the identification of Syringae Cortex, and can be added to the information contained in the Chinese Pharmacopeia 2015 edition. 相关文章 | 计量指标

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Anti-gastric ulcer effect of Brucea javanica oil injection in mice Qian Li;He Pan;Chen Liang;Qiujv Wang;Linlin Shi;Yuyang Zhang* 2013 (6):  162-169.  摘要 ( 462 )   This research is aimed at investigating the anti-gastric ulcer activity of Brucea javanica oil injection (BJO) on various gastric ulcer models in mice. Kunming mice (male or female, 18-22 g) were used as the subjects and divided into five groups. They were given normal 0.9% saline 10 ml/kg (n=10), cimetidine (regarded as the positive control) 200 mg/kg (n=10), BJO 0.2, 0.4 and 0.8 ml/kg (n=10, 10, 10). Four models with ulcer disease were established by water-immersion, ethanol, aspirin, and reserpine, respectively. The ulcer index were generally evaluated with macroscopic observation and scaled to determine the effects of the tested drugs. In addition, the pathological hematoxylin and eosin (HE) staining for histopathologic examination was utilized to further confirm the pesticide effect. The results displayed that BJO significantly improved the ulcer damage induced by waterimmersion, aspirin or reserpine and the satisfied effect was at the dose of 0.8 ml/kg, but the ulcer index of the ethanol-induced gastric ulcer had scarcely any significant improvement after BJO treatment when compared with the control group. Data of HE staining in aspirin and reserpine-induced ulcers showed that all the tested drugs had noteworthy protective effect in mice with gastric ulcer damage. The outcome of BJO treatment was superior to the one in the control, which testifies the therapeutic effect of BJO. It might be inferred that Brucea javanica oil injection is effective in treating a few of pathological models of mice with gastric ulcer on. Precise mechanisms of the action, however, need to be investigated in the near future. 相关文章 | 计量指标