亚洲传统医药

本期目录

2012年, 第7卷, 第5期　刊出日期：2012-10-20 上一期    下一期

Reviews

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Advances in the seed biology of Schisandra chinensis Haitao Cheng *;Jun Yina;Wei Sun;Jing Li;Xuesong Cao;Yonghua Xu;Hai Sun 2012 (5):  189-194.  摘要 ( 635 )   This paper reviews the latest information on the growth of Schisandra chinensis, its fruit and morphous, the characteristics of dormancy and storage and the germination of its seeds. A variety of factors, including the temperature, seed soaking, hormone pretreatment, chemical and the endogenesis inhibitory substances, are proposed as reasons for its low germination rate and ways are suggested to improve these. 相关文章 | 计量指标

Regular Articles

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Pharmacognostical studies on the root of Toddalia asiatica (L.) Lam. (Rutaceae) Varadharajan Madhavan;Sunkam Yoganarasimhan *;Poonam Shah;Magadi Gurudeva;Rajamanickam Deveswaran 2012 (5):  195-203.  摘要 ( 772 )   Toddalia asiatica is an important medicinal plant used in the treatment of diseases like paralysis, malarial and intermittent fever, dyspepsia, colic, diarrhea, cough, bronchitis, nausea, wounds, ulcers, epilepsy, gonorrhea and general debility; it is known as Kanchana in Ayurveda and as Milagaranai in Siddha system of medicine. The present study provides taxonomic characters of the plant, pharmacognostical and phytochemical details of the roots which help in laying down pharmacopoeial parameters. The diagnostic characters like the presence of oil glands on the under surface of leaflets and oil canals in the cells of the secondary cortex and secondary phloem region, presence of rhomboidal crystals, sclerenchyma cells in secondary cortex and presence of starch grains in the secondary cortex, secondary phloem and medullary rays help in the identification of the drug. Preliminary phytochemical analysis revealed the presence of alkaloids, carbohydrates, phytosterols, fixed oils, tannins, flavonoids, volatile oil, gums and mucilage. HPTLC studies of alcohol and aqueous extracts help in the identification of the drug besides in isolating and identifying the biomarker compound. 相关文章 | 计量指标

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Quantitative evaluation of Renshenguipi Wan using a combination of capillary electrophoresis fingerprints and infrared spectrum fingerprints Guoxiang Sun*;Xiaowen Dou 2012 (5):  204-213.  摘要 ( 856 )   Two methods based on capillary electrophoresis fingerprints (CEFPs) and infrared spectrum fingerprints (IRFPs) were developed to quantitatively evaluate the quality of Renshenguipi Wan (RSGPW). The CE conditions, including the CE buffer system chosen using a triangle optimization method, were optimized using the chromatographic fingerprint resolution index (RF) as the objective function and according to actual separations achieved. The optimal CE buffer system consisted of 50 mmol/L borax-50 mmol/L disodium hydrogen phosphate (1:1, v/v), with a pH adjusted to 9.5 by phosphoric acid, and the baseline separation was achieved within 25 min at an applied voltage of 13 kV. A total of 13 common peaks were confirmed to be present in 10 batches of RSGPWs by choosing ferulic acid as the reference peak and the quality of RSGPW was determined by the system quantitative fingerprint method (SQFM) on the basis of CEFPs. All samples were extracted with methanol under the same conditions and the IRFPs of the extracts were recorded. Equal weigh integration of the two methods based on CEFPs and IRFPs was used for the quality control of RSGPW, which proved that CEFPs combined with IRFPs are better for evaluating the quality of RSGPW. 相关文章 | 计量指标

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Simultaneous determination of myricetin, quercetin, and kaempferol in Juglandis Mandshuricae Cortex by high-performance liquid chromatograph Ruijuan Du;Min Song;Xiaowen Lun;Jinfeng Li;Xiaohong Hou * 2012 (5):  214-219.  摘要 ( 778 )   A simple and reliable high-performance liquid chromatographic method was established for the simultaneous determination of myricetin, quercetin, and kaempferol in Juglandis Mandshuricae Cortex. The chromatographic separation was performed on a Kromasil C18 column (250 mm×4.6 mm, 5 μm) with a gradient elution of methanol and 0.2% aqueous formic acid at a flow rate of 1.0 ml/min. The detector wavelength was 370 nm and the column temperature was 30ºC. The linear ranges were from 3.93 to 62.88 μg/ml (r=0.9998), 3.80 to 60.80 μg/ml (r=0.9996), and 0.41 to 6.62 μg/ml (r=0.9997) for myricetin, quercetin, and kaempferol, respectively. The precision for myricetin, quercetin, and kaempferol was 1.8%, 0.4%, and 1.9%. The method recoveries were 101.0% for myricetin, 96.5% for quercetin, and 97.0% for kaempferol. The contents of the target analytes were (98.90±0.45) μg/g for myricetin, (54.60±0.30) μg/g for quercetin, and (7.20±0.07) μg/g for kaempferol. The results demonstrated that the developed HPLC method would be employed to control the quality of Juglandis Mandshuricae Cortex. 相关文章 | 计量指标

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Determination of total flavonoids content and quantification of rutin in Momordica tuberosa (Roxb) Cogn. fruits by RP-HPLC Maheshkumar S. Kale*;Kirti S. Laddha 2012 (5):  220-225.  摘要 ( 676 )   In present study, determination of the total flavonoid content and quantitative estimation of rutin in methanolic extract of Momordica tuberosa (Roxb) Cogn. fruits were performed. The spectrophotometric method based on the formation of complexes with aluminium chloride was applied to determine the total flavonoids content. Presence of rutin was identified using chemical test and thin layer chromatography (TLC). Quantification of rutin was done by RP-HPLC method using C-18 column (250 mm × 4.6 mm, 5μm) with an isocratic mobile phase, methanol: water: acetic acid (45:54:1 v/v/v) at a flow rate of 1.0 ml/min. The wavelength of maximum absorption (λmax) selected for quantification of rutin was 257 nm. The method was validated for accuracy, precision and specificity. Validation revealed that the method is specific, accurate, precise, reliable and reproducible. The regression data for the calibration plot showed a good linear relationship with r = 0.9994 for rutin. Limit of detection and limit of quantification were 2.8 and 9.0 μg respectively. Intra and inter-day RSD of retention times and peak areas was less than 1.9%. A recovery study was carried out to check the accuracy of the method at three different concentrations and average recovery was between 95.9 and 103.4% for rutin. Total flavonoid content and the amount of rutin present in M. tuberosa fruits were found to be 0.47% (w/w) and 0.27% (w/w), respectively. 相关文章 | 计量指标

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Pharmacognostical studies of the root of Capparis sepiaria L. (Himsra) - an ayurvedic drug Varadharajan Madhavan ;Sunkam Yoganarasimhan *;Magadi Gurudeva;Ajay Shankar Pandey;Deveswaran Rajamanickam 2012 (5):  226-235.  摘要 ( 783 )   Capparis sepiaria is known as Himsra in ayurvedic medicine and is used in the treatment of several diseases. The pharmacognostical evaluation of the root of C. sepiaria which is used for medicinal purposes is presented in this paper. The study includes the morphology of the plant, macro-, microscopical characteristics of the root, macerate elements, histochemical and UVstudies, physico-chemical constants and phytochemical analysis. The findings revealed the presence of druse-type crystals in cork cells, the presence of stone cells in many layers in the secondary cortex, the presence of abundant simple starch grains in the secondary cortex and in medullary ray cells, and the presence of a pericyclic stone cell layer which helps the identification of the drug. Preliminary phytochemical analysis revealed the presence of alkaloids, carbohydrates, phytosterols and flavonoids in the alcoholic extract while the aqueous extract contained alkaloids, carbohydrates, saponins, flavonoids, gums and mucilage. The petroleum ether extract contained phytosterols, fixed oils and fats. HPTLC finger print profiles of the aqueous and alcoholic extracts have been obtained which will be useful for the standardization of this drug and also help to isolate biomarker compounds. 相关文章 | 计量指标

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Isolation of bioactive phytoconstituents from Achyranthes aspera Linn. with anti-hyperlipidemic activity Brajesh K. Sharma;Deepa Iyer;Umesh K. Patil * 2012 (5):  236-245.  摘要 ( 710 )   Phytochemical investigation of the aerial parts of Achyranthes aspera Linn. resulted in the isolation of octyl octadec-9-enoate and heptacosan-14-β-ol for the first time from the plant. The structure of the component has been established on the basis of spectral data analysis. Phytochemical screening revealed the presence of glycosides, tannins and alkaloids in ethanolic extract of Acyhranthes aspera while chloroform fraction revealed the presence of sterols. The present study was undertaken to explore the antihyperlipidemic effect of ethanolic extract of of Achyranthes aspera, its chloroform fraction, octyl octadec-9-enoate and heptacosan-14-β-ol in Triton- induced hyperlipidemic rats. Animals were administered with i.p. injection of Triton WR 1339 at dose of 400 mg/kg body weight. After 24 h. of Triton administration the ethanolic extract, its chloroform fraction, octyl octadec- 9-enoate and heptacosan-14-β-ol were administered orally at doses of 200 mg/kg body weight in rats. The extract, fraction and heptacosan-14-β-ol inhibited the total cholesterol, triglycerides, low density lipoproteins level (LDL), and significantly increased high density lipoprotein level (HDL). The results obtained suggest marked antihyperlipidemic activity of heptacosan-14-β-ol compared to octyl octadec-9-enoate. 相关文章 | 计量指标